This is from last summer - but I just bumped into it.
A comprehensive list - many I know and remember fondly - many which escaped me till now (e.g. No 2 "Christian the Lion" - how had i missed that?).
It's hard to argue against the "Star Wars Kid" being at No 1.. but my favourite is still probably "David After Dentist" (No 7 - if you only watch one... watch that one).
A good list with useful contextual commentary too.
The 100 Most Iconic Internet Videos [Full List] - Urlesque.
